What this protocol does
This protocol automates assembly of CRISPR gRNA constructs on the Undergrad: it preps and anneals the guide oligos into a duplex insert, ligates the insert into a digested CRISPR vector, transforms competent cells, plates on selection, and screens colonies for correct constructs. Thermocycling, magnetic clean-up, and the ligation/transformation liquid handling run hands-free with camera checks on the bead steps.
Who it's for
Genome-editing and synthetic-biology labs building guide libraries or one-off constructs that want fast, reproducible gRNA cloning without the repetitive pipetting of anneal-ligate-transform across many guides.
The four-layer architecture at a glance
