Instruments
- Undergrad + Accuris AutoMATE 96 (96-channel fixed head)
- On-deck magnetic separation block
- Mini plate centrifuge and vortex (bead resuspension, quick spin-downs)
- 4°C cooling block for RNA and reagents
- Onboard cameras for vision verification
Input requirements
| Parameter | Target |
|---|---|
| Input | Crude IVT + DNase reaction, ~40–50 µL/well |
| Format | 96-well PCR plate, held at 4°C |
| Handling | Nuclease-free throughout; keep RNA cold |
Labware
- 96-well PCR plate (input) and a clean collection plate
- 50 mL conical reservoirs: bead slurry, fresh 70% ethanol, nuclease-free water
- Filter tips
- Waste container
Reagents
- Carboxyl-coated paramagnetic SPRI beads (room temperature, fully resuspended)
- Freshly prepared 70% ethanol (nuclease-free water + absolute ethanol)
- Nuclease-free water for elution
Make the 70% ethanol fresh. Ethanol absorbs water over time — above 70% it washes poorly (salt carryover), below 70% it strips RNA off the beads and tanks your yield. Keep everything nuclease-free: any RNase degrades the product and fails integrity QC.