Normalize input
The robot reads your input concentrations and dilutes each well to a uniform target (e.g. 20 ng in 30 µL), so every sample enters fragmentation on equal footing.
~10 minVision confirms tip pickup and well-by-well dispense.Fragment / tagment
Fragmentation or tagmentation mix is added and the plate is moved to the thermocycler for the timed enzymatic step. Fragment size is set by incubation time/temperature per your kit.
15–30 minEnd repair & A-tailing
End-repair and A-tailing enzymes are added directly and incubated, preparing fragments for adapter ligation.
~30 minAdapter ligation
Adapters and ligation mix are added; the robot mixes gently and incubates to ligate adapters onto both fragment ends.
~15 minPost-ligation SPRI cleanup
SPRI beads remove enzymes and adapter dimers. The magnet engages, supernatant is removed, two 80% ethanol washes follow, beads air-dry, and DNA is eluted.
~20 minVision verifies magnet engagement, supernatant clearance, and that beads aren't over-dried.Indexing PCR
Unique dual indexes and PCR master mix are added per well; the plate cycles on the thermocycler for the minimum cycles needed to preserve complexity.
~30 minDual-sided size selection
A two-ratio SPRI selection removes both large fragments and small adapter dimers, leaving the target insert range. The bead ratios are set to your desired size window.
~25 minVision checks each bead transfer and wash.Final elution
Final libraries are eluted into a clean plate, ready for quantification, the Bioanalyzer/TapeStation, and pooling.
~5 min