Library / Automated DNA Extraction (96-well) / Steps

Protocol steps

Executed hands-free. Volumes/timings are illustrative — match to your kit.

The four-layer architecture

Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.

1
IntentYou describe automated dna extraction (96-well) in plain language — no scripting.
2
ProtocolValidated into stages: Lyse & bind → Remove supernatant → Wash → Elute.
3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.

Steps, layer by layer

1Lyse & bind~10 min
Atomic actionsAdd lysis/binding buffer and magnetic beads to the sample plate; mix and incubate.
Vision-verified◉ Vision confirms dispense and mixing.
2Remove supernatant~5 min
Atomic actionsEngage the magnet and remove the supernatant to waste.
Vision-verified◉ Vision verifies magnet engagement and clearance.
3Wash~10 min
Atomic actionsRun the wash series, verifying clearance between washes.
4Elute~5 min
Atomic actionsElute purified DNA into a clean plate.