Common issues & fixes
| Symptom | Likely cause | Fix |
|---|---|---|
| Low yield | Insufficient lysis; bead loss | Extend lysis; check bead resuspension and volumes. |
| Poor purity (A260/230) | Carryover of chaotropic salts | Add/extend wash; ensure full supernatant removal. |
| Sheared DNA | Over-vigorous mixing | Reduce mix speed for HMW applications. |
| Run paused | Vision flagged an exception | Re-seat as prompted; resume. |
When not to automate this
For low-throughput needs (a few samples), manual spin columns may be simpler. For high-molecular-weight DNA (e.g. long-read sequencing), validate that the bead chemistry and mixing preserve fragment length.
Verification: this protocol is simulation-verified — confirmed to run in the simulator but not yet run on hardware by RoR. Validate before using with critical samples.