Instruments
- Undergrad + Accuris AutoMATE 96
- Magnetic module
- Onboard cameras enabled for vision verification
Input requirements
| Parameter | Target |
|---|---|
| Sample type | Cell or tissue lysate (kit-compatible) |
| Quantification | Fluorometric (e.g. Qubit) recommended |
| Handling | Nuclease-free; keep cold |
Labware
- Deep-well sample plate
- Elution plate
- Reagent reservoirs
- 200 µL filter tips
Reagents
- Lysis/binding buffer
- Magnetic binding beads
- Wash buffers 1 & 2
- Nuclease-free elution buffer
RNA is degradation-prone. Keep samples and the deck cold where possible, use nuclease-free consumables, and run promptly after lysis.