Library / Automated Viral RNA Extraction / Steps

Protocol steps

Executed hands-free. Volumes/timings are illustrative — match to your kit.

The four-layer architecture

Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.

1
IntentYou describe automated viral rna extraction in plain language — no scripting.
2
ProtocolValidated into stages: Inactivate & lyse → Bind → Wash → Elute.
3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.

Steps, layer by layer

1Inactivate & lyse~10 min
Atomic actionsAdd lysis/inactivation buffer (with carrier RNA) to primary samples and mix to inactivate and release viral RNA.
Vision-verified◉ Vision confirms dispense into every well.
2Bind~10 min
Atomic actionsAdd magnetic beads and incubate to bind viral RNA.
3Wash~10 min
Atomic actionsEngage the magnet and run the wash series, verifying clearance between washes.
Vision-verified◉ Vision verifies magnet engagement and supernatant removal.
4Elute~5 min
Atomic actionsElute purified viral RNA into a clean plate, ready for RT-qPCR.