What this protocol does
This protocol runs a complete 96-well sandwich ELISA on the Undergrad: plate coating, blocking, sample and antibody additions, wash cycles, substrate development, and the final read on your plate reader. The whole assay runs hands-free on a standard bench — no dedicated automation room.
Every liquid-handling step is vision-verified, so a mis-seated tip or a misaligned plate is caught mid-run rather than discovered later in your data. That's the difference between a failed plate you find out about at the reader and one the robot corrects in real time.
Who it's for
Labs running ELISAs at any regular cadence — screening, QC, or research — that want to free scientists from the repetitive pipetting and wash cycles without buying a dedicated liquid handler or redesigning the bench.
The four-layer architecture at a glance
