1
Coat
The robot dispenses capture antibody into all 96 wells and starts the coating incubation. Vision confirms full-column coverage before incubating.
2
Wash & block
Three wash cycles with PBST, then blocking buffer across the plate. Aspirations are checked for complete well clearance between cycles.
3
Add samples & standards
Samples and the standard curve are dispensed to their mapped wells with fresh tips per transfer to prevent carryover.
4
Detect
Detection antibody is added, incubated, and followed by another wash series to remove unbound conjugate.
5
Develop & read
Substrate is added and stopped after the timed development, then the plate moves to the reader for absorbance at 450 nm.