Expected output
A ready-to-cycle qPCR plate with tight technical replicates. Eliminating manual pipetting variation typically tightens Cq standard deviation across replicates versus hand setup.
QC metrics & targets
| Metric | What good looks like |
|---|---|
| Replicate Cq SD | Lower than manual baseline |
| No-template controls | Clean — no carryover |
| Standard curve | Efficiency & R² in spec |
Vision verification: fresh-tip pickup is confirmed before each template transfer, so cross-contamination — the classic qPCR failure — is designed out.