The four-layer architecture
Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.
1
IntentYou describe automated qpcr reaction setup in plain language — no scripting.
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2
ProtocolValidated into stages: Distribute master mix → Add template → Seal & cycle.
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3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
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4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.
Steps, layer by layer
1Distribute master mix~10 min
Atomic actionsDispense qPCR master mix into every reaction well.
Vision-verified◉ Vision confirms even dispense across the plate.
2Add template~20 min
Atomic actionsAdd template/sample to each well using fresh tips per transfer to prevent carryover.
Vision-verified◉ Vision verifies fresh-tip pickup per transfer.
3Seal & cycle~5 min
Atomic actionsPrompt for optical sealing, then move the plate to the thermocycler for cycling.