Expected output
A pooled, indexed library spanning up to a full plate of samples, with consistent representation. Automating tagmentation and pooling removes the per-sample pipetting variation that drives uneven read distribution in high-plex runs.
QC metrics & targets
| Metric | What good looks like |
|---|---|
| Index balance | Even representation across samples |
| Insert size | Tight, in target window |
| Pool yield | Sufficient for sequencing in spec |
| Adapter dimers | Minimal after cleanup |
Vision verification: the tagmentation transfers, pooling, and bead cleanup are all camera-confirmed, so an under-pooled or mis-indexed sample is caught before it skews the run.