Instruments
- Undergrad + Accuris AutoMATE 96
- Magnetic module
- Thermocycler (on-deck or adjacent)
- Onboard cameras for vision verification
Input requirements
| Parameter | Target |
|---|---|
| Input | Low-input DNA per kit spec (normalize first) |
| Plex | Up to full-plate, unique dual indexes |
| Quantification | Fluorometric before normalization |
Labware
- Normalized input plate
- Index plate
- Reagent reservoirs / cold block
- PCR plate
- SPRI bead + ethanol troughs
- Filter tips
Reagents
- seqWell-style tagmentation reagents
- Unique dual indexes + PCR master mix
- SPRI beads, fresh 80% ethanol, elution buffer
Make 80% ethanol fresh the day of the run, and keep enzymes on the cold block. Low-input prep is unforgiving of evaporation and over-dried beads.