The four-layer architecture
Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.
1
IntentYou describe automated seqwell library prep in plain language — no scripting.
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2
ProtocolValidated into stages: Normalize input → Tagment → Index PCR → Pool → SPRI cleanup → Elute.
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3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
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4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.
Steps, layer by layer
1Normalize input~15 min
Atomic actionsDilute each well to the kit's target input so all samples tagment equivalently (see the Plate Normalization protocol).
Vision-verified◉ Vision confirms per-well dispense.
2Tagment~20 min
Atomic actionsAdd tagmentation mix and incubate to simultaneously fragment and tag the DNA.
3Index PCR~40 min
Atomic actionsAdd unique dual indexes and master mix; amplify on the thermocycler for the minimum cycles needed.
4Pool~15 min
Atomic actionsCombine indexed libraries into a single pool per plate.
Vision-verified◉ Vision verifies each pooling transfer.
5SPRI cleanup~25 min
Atomic actionsBead-clean the pool: magnet, two 80% ethanol washes, brief dry, and elute.
Vision-verified◉ Vision verifies magnet engagement, washes, and that beads aren't over-dried.
6Elute~5 min
Atomic actionsElute the final pooled library, ready for QC and sequencing.