What this protocol does
This protocol automates routine passaging of adherent cells on the Undergrad: it images the plate to assess confluency, aspirates spent media, washes, adds trypsin and incubates to detach cells, neutralizes and collects the suspension, seeds a new plate at the target density, and returns the culture to the incubator. Vision keeps density and detachment consistent from passage to passage.
Who it's for
Cell-biology and translational labs maintaining adherent lines that want consistent, audit-ready passaging without the variability and hands-on time of manual splits — especially for keeping cultures healthy across nights and weekends.
The four-layer architecture at a glance
