The four-layer architecture
Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.
1
IntentYou describe automated cell culture passaging in plain language — no scripting.
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2
ProtocolValidated into stages: Assess confluency → Aspirate media → Wash cells → Add trypsin → Neutralize & collect → Seed new plate → Incubate & monitor.
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3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
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4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.
Steps, layer by layer
1Assess confluency~3 min
Atomic actionsImage the plate and measure confluency to decide whether passaging is needed.
Vision-verified◉ Vision/imaging estimates confluency and flags wells outside the target range.
2Aspirate media~5 min
Atomic actionsRemove spent media from each well to waste.
Vision-verified◉ Vision confirms full aspiration without touching the cell layer.
3Wash cells~5 min
Atomic actionsAdd wash buffer (PBS) to remove residual media and serum, then aspirate.
4Add trypsin~8 min
Atomic actionsAdd dissociation reagent and incubate to detach the cells from the surface.
Vision-verified◉ Vision watches for cell rounding/detachment before proceeding.
5Neutralize & collect~5 min
Atomic actionsAdd serum-containing media to neutralize the trypsin and collect the cell suspension.
6Seed new plate~8 min
Atomic actionsDispense cells into a new plate at the target seeding density.
Vision-verified◉ Vision confirms even dispense across the destination plate.
7Incubate & monitorvaries
Atomic actionsReturn the new plate to the incubator and monitor growth.