Common issues & fixes
| Symptom | Likely cause | Fix |
|---|---|---|
| Incomplete detachment | Trypsin time too short or cold reagents | Extend the incubation slightly; pre-warm reagents; confirm rounding on camera. |
| Low viability | Over-trypsinization | Shorten trypsin exposure; neutralize promptly with serum media. |
| Uneven seeding density | Poor suspension mixing | Add a mix step before dispense; confirm even dispense on camera. |
| Contamination risk | Non-sterile handling | Run in an appropriate enclosure; use sterile consumables; keep media capped when idle. |
When not to automate this
This protocol is currently simulated — validate viability and density against your manual passage baseline before relying on it. For sensitive primary cells or non-standard dissociation, confirm the line tolerates automated handling first.
Verification: this protocol is simulation-verified — confirmed to run in the simulator but not yet run on hardware by RoR. Validate before using with critical samples.