Instruments
- Undergrad + liquid-handling module
- Integrated or adjacent incubator
- Plate reader / imaging for confluency (optional)
- Onboard cameras enabled for vision verification
Input requirements
| Parameter | Target |
|---|---|
| Cell type | Adherent lines (line-specific dissociation) |
| Confluency target | Set per line (e.g. passage at ~80–90%) |
| Split ratio | Per your validated SOP |
Labware
- Cell culture plates or flasks (kit-compatible)
- Reagent reservoirs
- Sterile tips
- Waste container
Reagents
- Growth media (pre-warmed)
- PBS or wash buffer
- Trypsin/EDTA (or comparable dissociation reagent)
- Media with serum for neutralization
Sterility is critical. Run in an appropriate biosafety enclosure, keep media pre-warmed, and minimise trypsin exposure time to protect cell viability. Confluency thresholds and split ratios are line-specific — set them to your validated SOP.