Library / Automated Plasmid Miniprep (96-well)

Automated Plasmid Miniprep (96-well)

Hands-free magnetic-bead plasmid purification from bacterial cultures — a full plate at a time.

SimulatedSample prep⏱ ~1hBeginnerMagnetic module96 samples

What this protocol does

This protocol automates 96-well plasmid miniprep on the Undergrad using magnetic-bead chemistry: alkaline lysis of the bacterial pellet, binding of plasmid DNA to magnetic beads, two washes to remove proteins and salts, elution, and transfer of clean plasmid to a fresh plate. The bead washes — the step most prone to carryover and yield loss when done by hand — are camera-verified.

Who it's for

Molecular biology and synthetic-biology labs prepping plasmids at plate scale for screening, sequencing, or transfection that want reproducible yields without the repetitive hands-on bead handling.

The four-layer architecture at a glance

Four-layer architecture diagram for Automated Plasmid Miniprep (96-well): intent, semantic workflow, robot actions, and atomic actions.
Intent → semantic workflow → robot actions → atomic actions — every stage runs through the same four layers on the Undergrad.

In this protocol