Instruments
- Undergrad + liquid-handling module
- Magnetic module
- Plate shaker for resuspension/mixing
- Onboard cameras enabled for vision verification
Input requirements
| Parameter | Target |
|---|---|
| Sample type | Bacterial culture pellet or lysate (kit-compatible) |
| Copy number | High- or low-copy (adjust input volume) |
| Quantification | Fluorometric (e.g. Qubit) recommended |
Labware
- Deep-well culture/lysate plate
- Elution plate
- Reagent reservoirs
- 200 µL filter tips
Reagents
- Resuspension buffer (with RNase A)
- Lysis buffer
- Neutralization/binding buffer
- Magnetic binding beads
- Wash buffer (ethanol-based)
- Nuclease-free elution buffer
Alkaline lysis is time-sensitive — do not over-incubate the lysis step, and neutralize promptly to protect supercoiled plasmid. Keep ethanol wash reservoirs sealed to avoid evaporation.