The four-layer architecture
Every step below flows through the same four layers — you work at the top, in plain language; the Undergrad handles everything down to camera-verified motion.
1
IntentYou describe automated plasmid miniprep (96-well) in plain language — no scripting.
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2
ProtocolValidated into stages: Lyse → Bind → Wash (x2) → Elute → Transfer.
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3
Robot actionsEach stage becomes grouped operations: move, aspirate, dispense, incubate, read.
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4
Atomic actionsPrecise instrument-level moves — each confirmed on camera before the next.
Steps, layer by layer
1Lyse~10 min
Atomic actionsResuspend the bacterial pellet, add lysis buffer, and mix gently to release plasmid DNA; neutralize to precipitate debris.
Vision-verified◉ Vision confirms resuspension and even dispense across the plate.
2Bind~10 min
Atomic actionsAdd magnetic beads and mix; incubate so plasmid DNA binds to the beads.
Vision-verified◉ Vision confirms bead dispense and mixing.
3Wash (x2)~10 min
Atomic actionsEngage the magnet and run two ethanol-based washes, verifying clearance between each.
Vision-verified◉ Vision verifies magnet engagement and full supernatant removal without disturbing the pellet.
4Elute~5 min
Atomic actionsAdd nuclease-free elution buffer off-magnet and mix to release plasmid DNA from the beads.
5Transfer~5 min
Atomic actionsReturn to the magnet, settle, and transfer clean plasmid eluate to a fresh plate, leaving beads behind.
Vision-verified◉ Vision confirms the eluate transfer leaves the bead pellet behind.